Molecular HLA Typing

Immucor Lifecodes HLA Class I & Class II SSO Typing
Inno-Train HLA Ready Gene PCR-SSP
Inno-Train HLA Ready Gene for Disease-Associated Typing
Inno-Train Ready Gene Wipe Test
Inno-Train HLA FluoGene
Inno-Train SuBiTo HLA Sequence-Based Typing


Immucor Lifecodes HLA Class I & Class II SSO Typing

Immucor Lifecodes HLA products are based on Luminex xMAP technology. The Lifecodes HLA-SSO Typing kits utilise sequence-specific oligonucleotides (SSOs) to identify which human leucocyte antigen (HLA) alleles are present in a PCR-amplified sample. It is the set of SSOs employed that determines the ability to distinguish among the various alleles present in the PCR amplification. All assays are carried out in a single reaction vessel with the addition of a single hybridization reagent for each locus tested.

Luminex Key Features:

 

Lifecodes HLA SSO Typing Kits Key Features:


 

Inno-Train HLA Ready Gene PCR-SSP

HLA Ready Gene is a molecular biological product line for detection of blood group systems based on PCR-SSP, which presents the ideal complement to serological blood group typing. The modular kit design gives the freedom to choose which systems to use for maximum flexibility.

Inno-Train’s molecular SSP detection systems are based on the Polymerase Chain Reaction (PCR), which enables amplification of defined DNA sequences. After successful amplification, the genomic DNA target sequence is present in a detectable concentration. The term Sequence Specific Priming (SSP), describes a variant of PCR in which only the sequence of the primer at the 3‘ end is responsible for specification of the allele to be identified. For complete PCR SSP analysis, several amplifications are performed in parallel. Evaluation of the result is performed by agarose gel electrophoresis. Under UV light, the bands are visualised by intercalating ethidium bromide and can be photographed for documentation.

 

HLA Ready Gene Brochure

 

Inno-Train HLA Ready Gene for Disease Associated Typing

HLA Ready Gene kits for disease-association typing are based on the SSP technique. The primer mixes are pre-aliquoted and dried, and all kits include negative control and ReadyPCR Mix, containing cresol red, glycerine, dNTPs and PCR buffer. HLA Ready Gene kits include:

 


Inno-Train Ready Gene Wipe Test

Inno-Train’s Ready Gene Wipe Test enables the user to monitor for contamination with amplicons created by the Ready Gene product line on working areas. It can also be used as a contamination control in Inno-Train’s Ready Gene typing kits.

The primer mix contains the primer pairs that amplify the 430 bp HGH fragment (human growth hormone gene), which is also part of all internal control amplicons in Inno-Train’s Ready Gene typing kits.
 


Inno-Train HLA FluoGene

FluoGene is a new typing method which combines the speed of the PCR-SSP method with Endpoint Fluorescence detection, without the need for agarose gel electrophoresis. FluoGene can be automated easily and flexibly: in its basic configuration the system uses the FluoVista Analyser in addition to a standard thermal cycle and the level of automation can be increased by adding a small pipetting station or a complete liquid handling processor with integrated modules.

Benefits of the FluoGene System:

 FluoGene Brochure  

Inno-Train SuBiTo HLA Sequence-Based Typing

The SuBiTo system from Inno-Train is for high resolution HLA typing by DNA sequencing. After amplification of the HLA genes by PCR, the HLA alleles present are typed using a DNA sequencer. All systems have been validated using an ABI capillary sequencer. The method is based on “Sanger“ sequencing with fluorescent-marked dideoxynucleotides.

The complete SuBiTo sequencing method requires genomic DNA as its basic material and is divided into 5 focal working steps:

  1. PCR amplification of genomic HLA sequences
  2. Enzymatic purification of the PCR products
  3. Sequencing reactions (Cycle Sequencing)
  4. Purification of the sequencing products, precipitation
  5. Sequencing (automated), detection and analysis of the DNA chains

 SuBiTo Brochure

Back to Product Index

 

 

Molecular HLA Typing Product List

CodeDescriptionPack Size
Lifecodes SSO Typing Kits & Accessories
628911 HLA-A Standard Kit (Including Taq) 50 Tests
628913 HLA-A eRES Kit (Including Taq) 50 Tests
628915 HLA-B Standard kit (Including Taq) 50 Tests
628917 HLA-B eRES Kit (Including Taq) 50 Tests
628921 HLA-C eRES Kit (Including Taq) 50 Tests
628923 HLA-DRB1 Standard Kit (Including Taq) 50 Tests
628925 HLA-DRB1 eRES Kit (Including Taq) 50 Tests
928927 HLA-DRB3/4/5 Kit (Including Taq) 50 Tests
628929 HLA-DQB1 Kit (Including Taq) 50 Tests
628933 HLA-DQA1 Kit (Including Taq) - Research Use Only 20 Tests
628937 HLA-DPB1 Kit (Including Taq) - Research Use Only 20 Tests
628941 HLA Null Allele Kit (Including Taq) - Research Use Only 50 Tests
628075 Taq Polymerase 25µl
628511 Streptavidin/PE 85µl - Sufficient for 100 HLA Tests
628155 DNA Kit Dilution Solution 9.9ml
757003 Nuclease Free Water 20ml
Ready Gene PCR-SSP
002050040 HLA-Ready Gene A - 24 Reactions per Test 40 Typings - 4 per Plate
002051020 HLA-Ready Gene B - 48 Reactions per Test 20 Typings - 1 per Plate
002052040 HLA-Ready Gene C - 48 Reactions per Test 40 Typings - 4 per Plate
002053010 HLA-Ready Gene ABC - 96 Reactions per Test 10 Typings - 1 per Plate
002054010 HLA-Ready Gene ABDR - 96 Reactions per Test 10 Typings - 1 per Plate
002055040 HLA-Ready Gene DR - 24 Reactions per Test 40 Typings - 4 per Plate
002056048 HLA-Ready Gene DQ - 8 Reactions per Test 48 Typings - 4 per Plate
002057030 HLA-Ready Gene DRDQ - 32 Reactions per Test 30 Typings - 3 per Plate
002067020 HLA-Ready Gene DPB1 – 48 Reactions per Test 20 Typings - 2 per Plate
Axitaq
GX090250 Axitaq 250 units
GX090251 AxiTaq 2 x 250 units
GX090252 AxiTaq 4 x 250 units
GX090254 AxiTaq 100 units
GX090257 AxiTaq 1000 units
GX090258 AxiTaq 5000 units
Ready Gene Disease Association Kits & Reagents
002058032 HLA-Ready Gene B27 Low 32 Types
002058096 HLA-Ready Gene B27 Low 96 Types
002062032 HLA-Ready Gene B57 Low 32 Types
002059012 HLA-Ready Gene B5/B57 Cross 12 Types
002061032 HLA-Ready Gene Coeliac Disease 32 Types
002064032 HLA-Ready Gene Narcolepsy 32 Types
002065032 HLA-Ready Gene A*31 32 Types
Ready Gene Wipe Test – For contamination control monitoring with all Ready Gene kits
002063064 Ready Gene Wipe Test 64 Tests
Inno-Train HLA FluoGene
002070040 HLA-FluoGene A 40 Tests - 24 Reactions Per Test
002071020 HLA-FluoGene B 20 Tests - 48 Reactions Per Test
002072040 HLA-FluoGene C 40 Tests - 24 Reactions Per Test
002073010 HLA-FluoGene ABC 10 Tests - 96 Reactions Per Test
002074010 HLA-FluoGene ABDR 10 Tests - 96 Reactions Per Test
002075040 HLA-FluoGene DR 40 Tests - 24 Reactions Per Test
002076024 HLA-FluoGene DQ 24 Tests - 9 Reactions Per Test
002077030 HLA-FluoGene DRDQ 30 Tests - 32 Reactions Per Test
002078096 HLA-FluoGene B27 96 Tests - 1 Reaction Per Test
002079010 HLA-Fluogene DPB1 10 Tests - 48 Reactions Per Test
002079020 HLA-Fluogene DPB1 20 Tests - 48 Reactions Per Test
002080010 HLA-FluoGene DRDQDP 10 Tests - 88 Reactions Per Test
SuBiTo Kits & Accessories
002090025 HLA-SuBiTo A High Res - Amplification of Exon 1-4  25 Types
002091025 HLA-SuBiTo B High Res - Amplification of Exon 1-4  25 Types
002092025 HLA-SuBiTo C High Res - Amplification of Exon 1-4  25 Types
002093025 HLA-SuBiTo DRB1 High Res - Amplification of Exon 2  25 Types
002094025 HLA-SuBiTo DQB1 High Res - Amplification of Exon 2 and 3  25 Types
002095025 HLA SuBiTo DPB 25 Types
002290001 HLA-SuBiTo A Seq2 No1  10 Types
002290002 HLA-SuBiTo A Seq2 No2  10 Types
002290003 HLA-SuBiTo A Seq2 No3  10 Types
002290004 HLA-SuBiTo A Seq2 No4  10 Types
002290005 HLA-SuBiTo A Seq2 No5  10 Types
002290006 HLA-SuBiTo A Seq2 No6  10 Types
002293001 HLA-SuBiTo DRB1 Seq2 No1  10 Types
002293002 HLA-SuBiTo DRB1 Seq2 No2  10 Types
002293003 HLA-SuBiTo DRB1 Seq2 No3  10 Types
002293004 HLA-SuBiTo DRB1 Seq2 No4  10 Types
002293005 HLA-SuBiTo DRB1 Seq2 No5  10 Types
002293006 HLA-SuBiTo DRB1 Seq2 No6  10 Types
002294001 HLA-SuBiTo DQB1 Seq2 No1  10 Types
002294002 HLA-SuBiTo DQB1 Seq2 No2  10 Types
002294003 HLA-SuBiTo DQB1 Seq2 No3  10 Types
002294004 HLA-SuBiTo DQB1 Seq2 No4  10 Types
002294005 HLA-SuBiTo DQB1 Seq2 No5  10 Types
002294006 HLA-SuBiTo DQB1 Seq2 No6  10 Types
00209AB1400 96 Well Sequencing Trays  25
002096760090 Ethanol Resistant Seals  100
00209AB0558 Thermostable Seals  100
0020900SW HLA HiType Software – Main Licence  1