Molecular KIR Typing

Immucor Lifecodes KIR SSO Typing
Inno-Train Ready Gene PCR-SSP KIR

Natural killer (NK) cells are lymphocytes of the innate immune system that fight infection by producing cytokines and killing infected cells. Their functional activity is regulated by the interaction between their surface killer cell immunoglobulin-like receptors (KIR) and Class I HLA alleles expressed on the target cell. NK cells will contain more than one type of KIR on their surface and these receptors can be either activating OR inhibitory in nature, the latter recognizing MHC Class I particularly HLA-C molecules. The target cells get lysed, if the sum of the inhibitory KIR signals is weaker than that of the activating. This situation can lead to a Graft versus Leukaemia effect (GVL) in bone marrow or stem cell recipients with absent HLA class I determinants, reducing the risk of relapse. Combinations of variants of MHC class I and KIR correlate not only with the outcome of transplants but also with autoimmunity (e.g. rheumatoid arthritis, psoriasis), virus infections (e.g. HIV, EBV, HCV) and pregnancy complications.


Immucor Lifecodes KIR SSO Typing

Key Features:

 


Inno-train Ready Gene PCR-SSP KIR

Inno-train’s molecular SSP detection systems are based on the Polymerase Chain Reaction (PCR), which enables amplification of defined DNA sequences. An advantage of PCR-SSP analysis is quick performance with few working steps. Whereas other typing systems that integrate the PCR technique e.g. PCR-SSO or PCR-RFLP consist of three different working steps (amplification, specification and detection), the PCR-SSP method only requires two working steps (amplification and detection).
 

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Molecular KIR Typing

CodeDescriptionSize
Immucor Lifecodes Kit
545110 KIR Kit 20 Types
Inno-Train Ready Gene PCR-SSP Kit
002060040 Ready Gene KIR – 24 reactions per test 16 Types