Molecular KIR Typing
Natural killer (NK) cells are lymphocytes of the innate immune system that fight infection by producing cytokines and killing infected cells. Their functional activity is regulated by the interaction between their surface killer cell immunoglobulin-like receptors (KIR) and Class I HLA alleles expressed on the target cell. NK cells will contain more than one type of KIR on their surface and these receptors can be either activating OR inhibitory in nature, the latter recognizing MHC Class I particularly HLA-C molecules. The target cells get lysed, if the sum of the inhibitory KIR signals is weaker than that of the activating. This situation can lead to a Graft versus Leukaemia effect (GVL) in bone marrow or stem cell recipients with absent HLA class I determinants, reducing the risk of relapse. Combinations of variants of MHC class I and KIR correlate not only with the outcome of transplants but also with autoimmunity (e.g. rheumatoid arthritis, psoriasis), virus infections (e.g. HIV, EBV, HCV) and pregnancy complications.
- Amplified DNA can be analysed with up to 100 different SSO probes simultaneously
- Multiplex reactions reduce labour, consumables and assay time
- XY platform allows walk-away acquisition from 1 to 96 samples at a time
- Convenience of full automation, if required, through collaboration with Hamilton Robotics
Inno-train’s molecular SSP detection systems are based on the Polymerase Chain Reaction (PCR), which enables amplification of defined DNA sequences. An advantage of PCR-SSP analysis is quick performance with few working steps. Whereas other typing systems that integrate the PCR technique e.g. PCR-SSO or PCR-RFLP consist of three different working steps (amplification, specification and detection), the PCR-SSP method only requires two working steps (amplification and detection).